ABSTRACT
La cavidad bucal y el interior de la bolsa subgingival constituyen nichos ecológicos propicios para albergar microorganismos que podrían actuar como patógenos oportunistas, como el Staphylococcus aureus y enparticular S. aureus resistente a la meticilina (SARM). La detección temprana de portadores reviste importancia para la salud pública. El objetivo de nuestro trabajo fue determinar por métodos fenotípicos y genotípicos la meticilino resistencia de cepas de S. aureus aisladas de mucosa bucal y bolsa subgingival y bolsa subgingival de 102 pacientes con enfermedad gingivoperiodontal. Se observó una prevalencia de S. aureus en bolsa subgingival del 10,8 por ciento (n=11) y en mucosa bucal del 19,5 por ciento (n=20). Se obtuvieron 31 aislamientos de S. aureus de los cuales 13 fueorn mec A positivos y 18 eran mec A negativos. La detección del gen mec A por PCR se utilizó como método de referencia para comparar los resultados de métodos fenotípicos para determinar la resistencia a meticilina. La detección rápida y exacta de S. aureus por métodos microbiológicos fenotípicos y genotípicos es relevante para evaluar la colonización y prevenir la propagación del SARM.
Subject(s)
Humans , Male , Adolescent , Adult , Female , Middle Aged , Gingival Pocket/microbiology , Mouth Mucosa/microbiology , Periodontitis/microbiology , Staphylococcus aureus/isolation & purification , Staphylococcus aureus , Staphylococcus aureus/genetics , Methicillin Resistance , Microbial Sensitivity TestsABSTRACT
The present study was carried out to compare the subgingival plaque microflora in mango leaf users. Fifty subjects of both sexes, 25 of them used tooth brush and 25 used mango leaf as their home care hygiene device were included in the study. The microbiological evaluation for specific bacterial counts of Actinobacillus actinomycetemcomitans, Prevotella intermedia, Porphyromonas gingivalis, Fusobacterium nucleatum and Peptostreptococcus micros were carried out for all subject. Specific microbial evaluation revealed significant decrease in the proportion of P. intermedia and P. gingivalis in mango leaf users compared to tooth brush users. It shows that mangiferin possesses antibacterial activity in vivo against specific periodontal pathogens such as P. intermedia and P. gingivalis. Use of mango leaf in conjunction with a tooth brush will be a good home care device for maintenance of oral hygiene.
Subject(s)
Evaluation Studies as Topic , Female , Gingival Pocket/microbiology , Humans , Male , Mangifera/chemistry , Oral Hygiene/education , Periodontal Diseases/microbiology , Periodontal Index , Plant Preparations/pharmacology , Plants, Medicinal , Porphyromonas gingivalis/drug effects , Prevotella/drug effectsABSTRACT
The present study was performed to evaluate the effect of one week metronidazole treatment on patients with advanced periodontal disease. Thirteen patients [three males and ten females] were selected in the study. Clinical and microbiological evaluations were carried out initially at the pretreatment visit, then repeated at 2 and 15 weeks after metronidazole treatment. Clinical measurements of pocket depth and attachement level was done using Ramfjord procedures [1967] [12]. In addition, microbiological samples were obtained from the most severely involved sites as shown by X-ray picture to detect the effect of metronidazole on anaerobic and facultative organisms. The results of this study revealed an improvement in clinical parameters as indicated by reduction in pocket depth associated with an apparent gain of attachement. This suggested that metronidazole permitted a type of disease resolution at the base of deep pockets. In addition, the presentinvestigation revealed that metronidazole eliminated anaerobic organisms from subgingival microbiota and reduced some of the facultative organisms such as streptococcus mutans, streptococcus sangius and streptococcus salivarius. These findings emphasize that the elimination of anaerobic organisms was responsible for the improved periodontal health; whereas the suppression but not the elimination of facultative species in the plaque were not primarily responsible for signs of disease observed. In addition, it may suppress the pathogenic flora so that when the antimicrobial therapy stops, a non pathogenic flora may reestablish in the subgingiral area. The metronidazole unique spectrum of activity against anaerobes involved in periodontal disease coupled with favorable clinical response to the short term treatment inelicate that this agent will have valuable usage in dentistry. However, long-term studies are needed to disclose if discontinuation of metronidazole therapy will lead to reestablishment of a subgingival microbiota inductive to progressive periodontal disease